Figure 5
From: Identification and functional characterization of Toll-like receptor 2–1 in geese
Expression of goose TLR2-1 in HEK293 cells results in NF-κB activation after stimulation of cells with MALP-2 or LPS. Cells were transfected with NF-κB firefly luciferase reporter and normalizing plasmid alone or with constructs expressing goose TLR2-1. After 16 hours, medium was replaced either with fresh medium alone or medium containing MALP-2 (2001) at 50 ng/ml or LPS at 20 ng/ml. NF- κB activation levels were measured after six hours by a dual luciferase reporter assay. The data are presented as mean +/− SD, and statistical analysised by One-Way Anova.